MiroSynth™ Cell Free DNA
Built Without Bacteria
By removing bacterial cloning from the process, MiroSynth™ helps simplify how DNA is sourced, built, and used across demanding applications. Ribbon Bio’s MiroSynth™ Cell-Free DNA delivers:
- Sequence-verified, linear DNA ready-to-use
- High complexities and accuracy
- No cloning, bacterial constraints, and workflow delays.
Traditional DNA Workflows
Requires bacterial cloning
Risk of contamination
Multi-step, time-consuming
Sequence space limitations
MiroSynth™
Fully cell-free
Defined, cleaner input
Ready-to-use, accelerated workflows
Greater design flexibility
Plasmid grade performance, without tradeoffs.
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Engineered for Accuracy
MiroSynth™ Cell-Free DNA combines algorithm-driven design, enzymatic assembly, and NGS-based validation to produce highly accurate, full-length DNA molecules.
- ≥90% of sequence-correct molecules across constructs up to 12 kb.
- Accuracy is maintained across increasing sequence length and complexity.
Standard
Plus
Ultra
Length
MiroSynth™ Cell Free DNA
700 - 8,000 bp
8,001 - 12,000 bp
Complexities
Global GC Content
35 – 65%
30-35%, 65 – 70%
20-30%, 70-80%
Local GC Content
25 – 75%
20-25%, 75 – 82%
10-20%, 82-90%
Internal A or T Homopolymers
≤ 20 bp for A/T
21-40 bp for A/T
Internal G or C Homopolymers
≤ 8 bp for G/C
9-12 bp G/C
13-16 bp G/C
Terminal A or T Homopolymers
≤ 20 bp for A
≤ 20 bp for T
21-40 bp A
21-40 bp T
41-130 bp A (3'-tail only)
41 - 130 bp for T (5'-head only)
Terminal G or C Homopolymers
≤ 8 bp for G/C
9-16 bp G/C
Short Repeats
≤ 40 bp for 2 – 4 bp repeats
≤ 56 bp for 5 – 8 bp repeats
> 40 bp, 56 bp repeats
Long Repeats
≤ 220 bp for 9 – 24 bp repeats
> 220 bp for 9 – 24 bp repeats
Accuracy
MiroSynth™ Cell Free DNA
≥ 90% sequence-correct molecules at any length
Why choose MiroSynth™
Ribbon Bio’s MiroSynth™ Cell-Free DNA delivers:
Sequence-verified, linear DNA ready-to-use
High complexities and accuracy
No cloning, bacterial constraints, and workflow delays
Intended Use
For research use only. Not for use in diagnostic procedures.
Unless otherwise agreed to in writing, Ribbon Bio does not intend these products to be used in clinical applications and does not warrant their suitability for any clinical diagnostic use. Customer is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.